Extended Data Fig. 3: Hsd3b6∆skin mice are resistant to skin infection with S. aureus and topical testosterone increases S. aureus infection.
From: Skin androgens regulate Staphylococcus aureus pathogenicity via quorum sensing
Images related to Fig. 1g and j. a, Male Hsd3b6fl/fl (n = 10) and Hsd3b6Δskin (n = 5) mice epicutaneously infected for 4 days with 1×106 CFUs of MRSA SAP430 (MRSA::lux). (i) Images related to Fig. 1g. (ii) Representative histology (H&E stain) and mean epidermal thickness (μm) ± SEM. Scale bars: 220 μm. *p < 0.05, by Mann-Whitney U-test (two-sided). Means ± SEM are plotted. (iii) Representative images showing skin lesions on day 4 post-infection. b, Female Hsd3b6∆skin mice were epicutaneously challenged with 1×106 CFUs with bioluminescent MRSA SAP430 (MRSA::lux) for 4 days with or without testosterone (vehicle, n = 3; testosterone n = 4). (i) Images related to Fig. 1j. (ii) Representative histology (H&E stain) and mean epidermal thickness (μm) ± SEM. Scale bars: 220 μm. ns, p ≥ 0.057 by Mann-Whitney U-test (two-sided). Means ± SEM are plotted. (iii) Representative images showing skin lesions on day 4 post-infection. c, Repeat of Female Hsd3b6∆skin mice were epicutaneously challenged with 1×106 CFUs with bioluminescent MRSA SAP430 (MRSA::lux) for 3 days with or without testosterone (vehicle, n = 5; testosterone, n = 5). Means ± SEM (error bars) are plotted. **p < 0.001 by two-way ANOVA. (i) Representative bioluminescence imaging (BLI) of mice at days 1, 2, and 3 post-infections. (ii) Quantification of infection progression over days.
