Extended Data Fig. 4: Testosterone stimulates quorum sensing readouts in S. aureus, but pregnenolone does not activate quorum sensing.
From: Skin androgens regulate Staphylococcus aureus pathogenicity via quorum sensing
a-d, Transcriptomics (RNA-seq) of HG003 strain of S. aureus treated through the early-log phase (OD600 0.4) with 10 nM or 100 nM testosterone, 10 nM pregnenolone, or vehicle alone. Differential gene expression was analysed using DESeq2 (two-sided Wald test) with Benjamini-Hochberg correction for multiple comparisons (FDR). a, Volcano plot demonstrating genes fold change in expression in testosterone transcriptome compared to vehicle treated S. aureus, (n = 3) as shown in Fig. 2a. Here, additional significant genes with lower fold-change are labelled. b, Volcano plot demonstrating change in expression with 100 nM of testosterone transcriptome compared to vehicle treated S. aureus, (n = 3). c, Volcano plot demonstrating change in expression with 10 nM of testosterone transcriptome compared to pregnenolone treated S. aureus, (n = 3). d, Volcano plot comparing the transcriptome of S. aureus treated with pregnenolone compared to vehicle control (n = 3).
