Fig. 2: Neutralization of spike S1 by nanodecoys.
a, Dose-dependent neutralization of spike S1 by LSC- or HEK-nanodecoys. Data are shown as mean ± SD, n = 3 independent experiments. b, Schematic illustrating the experimental design. c, Interaction of spike S1 (red) and nanodecoys (white) when co-cultured with lung cells (green). d, Schematic illustrating the experimental design. e, Representative confocal images showing internalization of nanodecoys by macrophages (CD4, red). Four images were taken. f,g, Schematic illustrating the co-culture experiment (f) and confocal images (g) showing the internalization of nanodecoys by macrophages co-cultured with lung cells (CD90, green). h–k, Flow cytometry analysis showing internalization of DiD-labelled nanodecoys by LSCs (h,i) and macrophages (j,k) and the corresponding quantitation (l). FSC-H, forward scatter height. PBS was used as a control group for LSCs (i) and macrophages (j). See Supplementary Fig. 18a for gating strategies. Data are shown as mean ± SD, n = 3 independent experiments. Statistical analysis was performed with two-tailed Student’s t test. Scale bars in c, e and g, 50 μm. Cartoon pictures in b, d and f were created with BioRender.com.
