Fig. 5: Nanodecoy inhalation accelerates the clearance of the SARS-CoV-2 mimic viruses in a mouse model.
a, Schematic showing the animal study design. Created with BioRender.com. b, Representative ex vivo IVIS imaging of lung tissues from mice with various treatments. n = 3 animals per group. Colour scale shows radiant efficiency (same units as c). c, Quantification of fluorescence intensities of SARS-CoV-2 mimics from the imaging data in b. Data are shown as mean ± SD, n = 3 animals per group. Statistical analysis was performed by two-way ANOVA with a Tukey post hoc test for multiple comparisons. p, photons. d, Representative confocal images of AF647-labelled SARS-CoV-2 mimics (red) in lung sections. Scale bar, 50 μm. n = 3 animals per group. Three images were taken for each animal. e, Corresponding semiquantitative analysis of AF647-labelled SARS-CoV-2 mimics in lung tissues. Data are shown as mean ± SD, n = 3 animals per group. Statistical analysis was performed by two-way ANOVA with a Tukey post hoc test for multiple comparisons. f–j, Cytokine array analysis of various inflammatory cytokines (j) in the serum 3 d after treatment: control (f), LSC-nanodecoy (g), SARS-CoV-2 mimic (h) and SARS-CoV-2 mimic plus LSC-decoy (i). IFN-γ, interferon γ; IL, interleukin; M-CSF, macrophage colony-stimulating factor; POS, positive control; TNF-α, tumor necrosis factor α.
