Fig. 6: LSC-nanodecoy inhalation treats SARS-CoV-2 infection in cynomolgus macaques.
a, Schematic depicting the cynomolgus macaque study design. IN, intranasal. IT, intratracheal. Created with BioRender.com. b,c, Viral sgRNA copies/swab in NSs (b) and BAL (c) at various time points following challenge. Each dot represents data from one animal. n = 3 animals per group. d, Representative H&E images of fixed lung tissues from SARS-CoV-2-infected cynomolgus macaques. At least three images were taken per animal. Scale bars, 500 μm (top), 100 μm (bottom). e, Representative images of SARS-N IHC staining in fixed lung tissues from SARS-CoV-2-infected cynomolgus macaques treated with control or LSC-nanodecoys 8 d post-viral challenging. n = 3 animals per group. Three images were taken for each animal. Scale bars, 100 μm (top), 20 μm (bottom). f, Quantification of lung fibrosis of infected cynomolgus macaques by Ashcroft scoring; each dot represents data from one animal. Data are shown as mean ± SD, n = 3 animals per group. Statistical analysis was performed by two-tailed Student’s t test. Ashcroft scoring was performed blindly. g, Quantitation of positive SARS-N numbers in lung tissues of infected cynomolgus macaques. Each dot represents data from one animal. Data are shown as mean ± SD, n = 3 animals per group. Statistical analysis was performed by two-tailed Student’s t test. h, Representative images of RNAscope in situ hybridization detection of vRNA in infected cynomolgus macaques. Zika served as a control probe. n = 3 animals per group. Three images were taken for each animal. Scale bar, 100 μm. i, Representative immunofluorescence images of SARS-N (red), pan-CK (green), Iba-1 (greyscale), CD68 (green), CD206 (magenta) and DAPI (blue). Scale bar, 50 μm. n = 3 animals per group. Three images were taken for each animal.
