Extended Data Fig. 2: CD therapy with BL@B-SA50.
(a) C57BL/6 mice were pre-sensitized by absorption of TNBS solution through the skin. After one week, TNBS solutions were slowly administered into the colon lumen of mice to induce CD. Then, mice were orally administered with Medium or B-SA (1.25 mg/kg), BL (2.5 × 108 CFU/kg), BL (2.5 × 108 CFU kg−1) + B-SA (1.25 mg/kg), BL@B-SA50 (BL: 2.5 × 108 CFU/kg, B-SA: 1.25 mg/kg) for four days. (b) Daily bodyweight development after administering TNBS solution into the colon lumen. Data were normalized as a percentage of the bodyweight at day 8. (c) Changes in DAI. (d) Lengths and (e) picture of mice colon with indicated treatments on day 13. Data are presented as mean ± s. d. from n = 5 biological replicates. Statistical analysis was calculated by unpaired Student’s two-sided t test. 1P, 2P, 3P, 4P and 5P, in (b, c) denotes the statistical significance of Health, BL@B-SA50, BL + B-SA, BL and B-SA group relative to the Control group, respectively. P in (d) denotes the statistical significance relative to the Control group. #P in (d) denotes the statistical significance of BL@B-SA50 relative to the Health group.
