Fig. 6: Characterization in adult rhesus macaque.
a, AAV in ex vivo cortical slice taken from a 14-year-old rhesus macaque. b, CAP-Mac is more efficient at transducing neurons in the grey matter of the cortex. c, Quantification demonstrates that CAP-Mac-delivered transgene produces more RNA but not DNA compared with AAV9-delivered transgene; n = 3 brain slices; two-tailed Welch’s t-test. d–f, AAV in adult rhesus macaques in vivo. d, Recovered DNA from adult macaque administered with an eight-capsid pool (7.5 × 1013 vg kg−1). Here n = 12 brain samples from two adult macaques. One-way analysis of variance using Tamhane’s T2 correction was tested against AAV9 enrichment. e, We intravenously injected 1 × 1013 vg of CAP-Mac packaging CAG-eGFP into a 17-year-old rhesus macaque via the saphenous vein to assess CAP-Mac protein expression in the adult macaque. f, CAP-Mac-mediated eGFP expression visualized after amplification with GFP antibody. Mean values ± s.e.m. are shown.
