Extended Data Fig. 5: Characterization of image resolution covered with biological tissues in varied NIR-II sub-regions. | Nature Nanotechnology

Extended Data Fig. 5: Characterization of image resolution covered with biological tissues in varied NIR-II sub-regions.

From: Fluorescence-amplified nanocrystals in the second near-infrared window for in vivo real-time dynamic multiplexed imaging

Extended Data Fig. 5

a, Typical SEM image of the PS beasds (~ 10 μm) used as the substrates for cubic-phase Tm3+-, Er3+- and Ho3+-based nanoparticles encapulation. Scale bar, 20 μm. b, Fabrication procedures for polystyrene (PS) beads tagged with single component (α-TmNPs, α-ErNPs or α-HoNPs). c, Schematic of in vitro imaging experiments using the DSNPs-tagged beads imaging that covered with biological tissues. The inset shows physical picture of mouse skull with a thickness of ~1 mm. d, e Fluorescence images of the three types of PS beads tagged with α-TmNPs (Tm-Ch, 1600–1700 nm), α-ErNPs (Er-Ch, 1500–1600 nm) and α-HoNPs (Ho-Ch, 1100–1200 nm), respectively, in the absence and presence of a covered slice of (d) mouse skin or (e) skull and corresponding image resolutions. The imaging resolutions were quantified by full width at half-maximum (FWHM) of a PS bead along the white-dased lines. Exposure time: 300 ms. Scale bar, 50 μm.

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