Extended Data Fig. 8: Noninvasive monitoring the specific labelling of elasitc fibres of arteries by α-Er@Af633 nanoprobes.
a, Time-line of monitoring of the α-Er@Af633 labeled elastic fibres. Free α-ErNPs without conjugation of Alexa Fluor 633 dyes were also adopted with identical experimental procedures as a control group. b, c, Real-time tracking metabolic processes of (b) α-Er@Af633 or (c) free α-ErNPs in vessel blood. Scale bar, 20 μm. Blood vessels with similar lumen diameters were monitored for comparing the metabolic behaviors of α-Er@Af633 or free α-ErNPs. d, e, Quantitative analysis of metabolic behaviors of (d) α-Er@Af633 and (e) free α-ErNPs in blood vessels by plotting the signal intensity along the whited dashed line in (b) and (c). Decreased fluorescence intensity of the vessel lumen was observed with injection of α-Er@Af 633 over time. f, Corresponding SBRs of the artery walls with injection of α-Er@Af 633 (from 3.0 to 4.4) or free α-ErNPs (from 2.8 to 1.3).
