Extended Data Fig. 9: Real-time dynamic multiplexed imaging of neutrophil behaviors in acute inflammation.
a, Multiplexed dynamic tracking of a single neutrophil (blue-dashed circle) migrating towards a small neutrophil cluster (white arrow) in the inflamed site in mice ear. The vascular profile was depicted by white dotted line. Scale bar, 20 μm. b, Migratory paths of the single neutrophil (presented as x-y projections). The calculated meandering index (MI) value of ~ 0.81 (>0.8) was commonly associated with chemotaxis, indicating its potential to be activated in inflammatory microenvironment. c, Real-time imaging of neutrophil extravasation (purple-dashed circle), which is a typical characteristic of activated state. Scale bar, 50 μm.
