Fig. 5: Variation of templating material and virus CPs.
a, The RNA–DNA hybrid origami (RNA-6HB) is obtained by thermally annealing 996 nt RNA with DNA staples. The poly(A) tail was left unfolded. b,c, The successful folding can be monitored by AGE (b), in both the ethidium bromide (EtBr) and the Alexa647 (A647) channel, and by AFM (c). d, Folded structures selected from AFM (left) and negative-stain TEM (right) images of RNA-6HB. The image dimensions correspond to 125 nm × 75 nm. e, EMSA for RNA-6HB showing a decrease in the electrophoretic mobility with increasing ε in both the EtBr and the A647 channel. f, Negative-stain TEM images (125 nm × 75 nm) of complexed RNA-6HB structures with ε = 500. g, Observed size distributions (in diameter) for plain RNA-6HB (blue) and RNA-6HB-500 (grey). h, NoVLPs (top, TEM image 150 nm × 150 nm) are disassembled into VP1 units and assembled with 6HB at ε = 500 (bottom). i, VLPs built from the major CP, VP1, of SV40 (top, TEM image 150 nm × 150 nm) are disassembled into their pentameric subunits and reassembled onto 24HB at ε = 5k (bottom, TEM images 200 nm × 100 nm). j, Pentamers formed from the major CP, VP1, of MPyV (top middle, TEM image 150 nm × 150 nm) can either be assembled into VLPs (top left, TEM image 150 nm × 150 nm) or complexed with DNA origami (top right). Development of a pentamer-based protein layer on 6HB (bottom left, TEM images 400 nm × 100 nm) or 24HB (bottom right, TEM images 200 nm × 100 nm) at ε = 1.25k.
