Extended Data Fig. 5: Proteomic analysis of Cho-PC in human serum and adsorption of LDL and HDL on NPs.
(a)The heatmap of apolipoproteins and complement proteins identified in PC when bare SNPs and cholesterol-precoated SNPs were incubated with the normal FBS. Cholesterol precoated SNPs were prepared by incubating bare SNPs in 5.1 mM cholesterol solution and subsequent three PBS washing. (b-e) The heatmaps (b, d) and relative protein abundance (c, e) of apolipoproteins (b, c) and complement proteins (d, e) identified in neat serum and protein corona on SNPs in human serum with different concentrations of cholesterol. (f-g) The amount of LDL (f) and HDL (g) adsorbed on SNPs by measuring the concentrations in supernatants before and after SNPs addition. The data are presented as mean ± standard deviation with n = 3 (biologically independent experiments).
