Fig. 5: Effect of FeSA@AH as a prophylactic for chronic alcohol intoxication.
From: Single-site iron-anchored amyloid hydrogels as catalytic platforms for alcohol detoxification
a, Schematic of the chronic alcohol intoxication model construction (Methods). Created with BioRender.com. b, Body weight changes in the four groups of mice during the feeding period. c, Representative H&E-stained images of liver in the four groups. d, Serum ALT and AST levels in mice. e, H&E images of colon (left part) and its assessed scores (right histogram) in different groups of mice. f, Immunofluorescence staining of the tight junction proteins in the colon (left part, 30× magnification). The tight junction proteins (Claudin-1, occludin and ZO-1) were stained green whereas the 4,6-diamidino-2-phenylindole (DAPI) was blue. The histograms (right) show the mean density of the normalized levels of occludin and ZO-1. IOD, integrated optical density. g, Taxonomic and phylogenetic tree of the top 21 most affected genera (genus with >10% mean abundance change in at least one group compared to others) by different treatments generated by GraPhlAn 4.0. Outer circles show the grouped mean relative abundance of each genus. h, Metabolic processes of alcohol to acetate and further in mice. The left colour blocks indicate the endogenous organs, liver, intestine and gut microbiota involved in alcohol decomposition, and the right shows the path in which FeSA@AH participated. The box-plot shows the relative levels of ko00770 pantothenate and CoA biosynthesis among groups (minimum–maximum). The heatmap shows 83 significantly changed pathways compared with those in the PBS group. Source data are provided as a Source Data file. i, LPS concentrations of mice in the four groups. Data are shown in the form of mean ± s.e.m. from n = 8 biological replicates. In c,e,f, the images displayed are representative of three independent biological replicates (n = 3), each producing consistent results. For histopathological, physiological and biochemical indexes (c–f,i), P values were tested by one-way analysis of variance followed by Tukey–Kramer test whereas the pairwise Wilcoxon test with Bonferroni–Holm correction was used for microbial taxa (h,i). *P < 0.05, **P < 0.01, ***P < 0.001.
