Fig. 2: Structure characterization of the UV cross-linked origami and its stability.
From: A DNA robotic switch with regulated autonomous display of cytotoxic ligand nanopatterns
a, Example of cryo-EM micrograph of the UV cross-linked origami structure. Scale bar, 100 nm. b, Representative 2D class averages of the UV cross-linked origami structure. c, Cryo-EM density map. d, Frames picked from the 3DFlex analysis. e, A 2% agarose gel electrophoresis of origami incubated in freshly prepared DMEM with 10% FBS at 37 °C. L, 1 kb DNA ladder; M, DMEM with 10% FBS; C, origami before adding to the medium. f, Normalized agarose gel intensity versus time for origamis. Data are presented as mean ± s.d., n = 2 independent measurements. g, Protrusion sites (coloured in blue) surrounding the origami for site-specific PEGylation. h, The PEGylation mechanism, via hybridization of ssDNA–PEG5K to 20 single-stranded protrusions of the origami. i, Electrophoresis of origamis in a 2% agarose gel. L, ladder; S, p8064 scaffold; UV, UV cross-linked origami, six-peptides-loaded device (pH_O6p) and PEGylated version (PEG_pH_O6p). j, Negative-stain TEM image of the PEG_pH_O6p. Scale bar, 100 nm. k, Two-dimensional class averages of the PEG_pH_O6p. Scale bar, 100 nm.
