Fig. 2: Characterizing and labelling polymersomes.
From: Polymersomes with splenic avidity target red pulp myeloid cells for cancer immunotherapy
a, Schematic representation of the four different polymersome topologies used in this study. b, Representative cryo-TEM micrographs of the four BODIPY-labelled polymersome topologies in their respective dialysis fluids, that is, SmS and LgS, were measured in Milli-Q water and SmT and LgT in 50 mM NaCl. Scale bars, 500 nm. Similar results for polymersome topologies were obtained in three independent experiments. c, Polymersome dimensions determined using the cryo-TEM micrographs. The heatmap indicates the corresponding polymersome aspect ratios. d, Schematic representation of the polymersome radiolabelling strategy. DFO-functionalized polymersomes were formulated by incorporating 5 wt% DFO-PEG24-PDLLA45 followed by incubation with radioactive 89Zr to yield 89Zr-polymersomes. e, Schematic representation of the polymersome fluorescent labelling strategy. BODIPY-polymersomes were formulated by incorporating 5 wt% BODIPY-PEG24-PDLLA45. f, Schematic representation of the polymersome stable isotype labelling strategy. N3-functionalized polymersomes were formulated by incorporating 5 wt% N3-PEG24-PDLLA45. These polymersomes were subsequently incubated with stable 157Gd complexed in DO3A-DBCO to yield 157Gd-polymersomes. All data are represented as mean ± s.d.
