Fig. 2: Establishing and screening a library of aNP-siRNA with diverse compositions.
a, aNP-siRNA library design space. N, nitrogen; P, phosphate. b, Compositions, identifiers, and characteristics of the aNP-siRNA formulations in the library. Formulations in bold and indicated by an asterisk (*) were selected for in vivo evaluation. c, Morphology and size were determined by cryo-EM analysis (see Supplementary Fig. 2 for extended cryo-EM data). Scale bars, 50 nm. d–f, Heatmaps represent formulation quality as assessed by percentage lipid aggregation quantified in cryo-EM images (d), shelf-life by percentage change in dispersity determined by dynamic light scattering after 28 days (e), and functional Fluc reporter gene silencing in vitro determined by bioluminescence measurements in RAW 264.7 dual-luciferase reporter cells after 48 h (f).
