Extended Data Fig. 1: aNP-siRNA formulation procedure. | Nature Nanotechnology

Extended Data Fig. 1: aNP-siRNA formulation procedure.

From: Nature-inspired platform nanotechnology for RNA delivery to myeloid cells and their bone marrow progenitors

Extended Data Fig. 1

Lipid molecules dissolved in ethanol are rapidly mixed via a T-junction with siRNA dissolved in sodium acetate buffer (25 mM, pH 4). The resulting intermediate product is then dialysed against PBS buffer (pH 7.4). Next, the intermediate product is rapidly mixed with apoA1 dissolved in PBS. The final product is filtered, concentrated, and analyzed.

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