Extended Data Fig. 5: Open-field and cylinder tests.
a, Experimental workflow for the behavior test and body weight examination. C57BL/6 mice aged 2 months were injected with RIDE (21.5 ng p24/side) or 1.5 μL PBS (control) into both sides of the striatum (n = 6/group). At 110 dpi, behavior tests were performed. b–d, Open-field test. Each experimental mouse was placed at the center of a white acryl square box (40 cm × 40 cm × 40 cm) in the dark. Spontaneous movements during a period of 5 min were monitored by digital video recording, and the travel distance (b), velocity (c), and time in the central area (d) were automatically tracked and analyzed by the software (Ethovision XT Version 15). e, Cylinder test. Mice were placed inside a glass cylinder with a diameter of 10 cm and a height of 25 cm. The number of times both the paws were placed on the cylinder wall during exploration was counted and recorded (3 min). f and g, Body weight changes for female and male mice post-intracerebral injection. n = 6 per group with three males and three females. Data and error bars represent mean ± SEM. n.s., not significant. Unpaired two-tailed Student’s t-tests were applied.
