Fig. 4: Distribution of RIDE and tissue health in the brain of WT mice after intracerebral injection. | Nature Nanotechnology

Fig. 4: Distribution of RIDE and tissue health in the brain of WT mice after intracerebral injection.

From: Customizable virus-like particles deliver CRISPR–Cas9 ribonucleoprotein for effective ocular neovascular and Huntington’s disease gene therapy

Fig. 4

a, Schematic illustration of IHC and H&E analysis of RIDE distribution and tissue health in the brains of mice. RIDE labelled by a GFP-encoding viral cassette (RIDE-Htt-GFP) was injected into the right striatum of the adult C57BL/6 mice at a dose of 125 ng p24. After 30 days, mice were killed and their brain tissues were collected for analysis. b,c, Distribution of RIDE-Htt-GFP in the mouse brain by immunohistochemistry analysis. The left striatum was non-injected. n = 1. The area of the black box in the whole brain (b) was enlarged and placed on the right (c). Scale bars, 1,000 μm (b), 200 μm (c, above) or 50 μm (c, below). d,e, Whole brain tissue analysis with H&E. n = 1. The area of the black box in the whole brain (d) was enlarged and placed on the right (e). Scale bars, 1,000 μm (d), 200 μm (e, above) or 100 μm (e, below). The area of the white box (c, above) is enlarged and placed at the bottom (c, below).

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