Extended Data Fig. 2: Characterization of the immune response to RIDE.
a–c, Type I immune response and RIDE transduction. Induction of IFNB1 (a), ISG15, (b) and RIG-I expression (c) in THP-1-derived macrophages by RIDE. IVT-gRNA (100 ng) was used as a positive control. **P = 0.0054 or 0.0058 for RIDE-100 ng or RIDE-300 ng versus IVT-gRNA (a). *P = 0.0211 or 0.0257 for RIDE-100 ng p24 or RIDE-300 ng p24 versus IVT-gRNA (b). *P = 0.0246 or 0.0256 for RIDE-100 ng or RIDE-300 ng versus IVT-gRNA (c). Data and error bars represent the mean ± SEM from three biologically independent replicates (a-c). d–f, Analysis of IgG in mice induced by RIDE. Mice were injected with 1 µg of p24 RIDE or Lenti-CRISPR via the footpads, and sera were collected for ELISA with Cas9- and p24- specific antibodies. NC, non-infected control, n = 4 mice (e) and 5 mice (f). LV, Lenti-CRISPR, n = 6 mice, RIDE, n = 7 mice. ***P < 0.0001 (e). NC versus RIDE, *P = 0.0394; NC versus LV, **P = 0.0035 (f). n.s., not significant. Data and error bars represent the mean ± SEM. Unpaired two-tailed Student’s t-tests.
