Extended Data Fig. 3: Characterization of the neuron tropism of hyRV-G envelope.
a, Illustration of rabies virus-derived hyRV-G envelope protein. The reconstructed hyRV-G contains the human IgE signal peptide, the extracellular and transmembrane domains of rabies virus glycoprotein, and the intracellular domain of VSV glycoprotein. b, The susceptibility of different cell lines and primary cells to the hyRV-G- and VSV-G-pseudotyped GFP-encoding IDLV. Samples were analyzed by flow cytometry. In total, 21.5 ng p24 IDLV was transduced into 1 × 105 cells. hyRV-G versus VSV-G, ***P < 0.0001, **P = 0.0019. c, In vivo characterization of the neuron specificity of hyRV-G-pseudotyped IDLV in the striatal region. In total, 21.5 ng p24 IDLV was injected. Representative images are shown from one of four mice. White arrowheads indicate the cells co-localized by the GFP signal and cell marker. Scale bar, 50 μm. d, Statistical analysis of GFP+ cells in different cell populations. Data and error bars represent mean ± SEM from three biologically independent replicates (b, d). Unpaired two-tailed Student’s t-tests.
