Fig. 6: Autologous MW-TMPs effectively enhance the antitumour immune response in human MPE.
a, Schematic illustration of the treatment process of MPE from patients with lung cancer, created in BioRender. Y, Z. (2025) https://BioRender.com/lgc5c9p. b, FCM analysis of the cell composition within human MPE (n = 5). c, Representative immunofluorescence images showing uptake of autologous MW-MPs (n = 5). Scale bars, 100 μm for the wider perspective. Scale bars, 50 μm for the smaller perspective. d, The proportion of cells taking up autologous MW-MPs in MPE (n = 5). e, Cellular composition of cells taking MPs (n = 6). f, WB analysis of the expression of CRT (n = 5). g, The concentrations of ATP in the supernatant of MPE cells (n = 3). h–o, The ratios of CD80+ DCs (h), the proportions of M1-like (i), the ratios of M1/M2 (j), the cytokine levels of Foxp3 (k) and IFNγ (l) in CD4+ T cells, the proportions of CD69 (m) and CD25 (n) in CD8+ T cells in MPE and the levels of NKG2D in NKT cells (o) (n = 5). p, The representative illustration of 3D spherical models constructed by MPE cells (n = 3). Scale bar, 200 μm. q, The representative images of the spherical size after 6-day stimulation and live/dead fluorescence staining of 3D spherical models (n = 3). Scale bars, 200 μm. r, The volume curve of 3D spherical models (n = 3). s, The statistical analysis of live/dead fluorescence staining of 3D spherical models (n = 3). t, Schematic illustrating the establishment and treatment of zebrafish PDX models, created in BioRender. Y, Z. (2025) https://BioRender.com/0g7gooc. u, The representative images and fluorescence intensity analysis of tumour cells in PDX models (n = 10). Scale bar, 50 μm. The data in d–q, t, u and w are shown in analytic plots as mean ± s.d., and ordinary one-way ANOVA was used for data in d, f–q and u. Two-way ANOVA was used for e and t. The exact P value was noted.
