Fig. 2: Lipidomics imaging of molecular replicas.
From: Nanoneedles enable spatiotemporal lipidomics of living tissues
a, Schematics of the nanoneedle imprinting system. Created with BioRender.com. b, Photographs illustrating the system approaching (top) and imprinting (bottom) nanoneedles on tissue. c, Scanning electron microscopy images of the molecular adsorbate on flat and nanoneedle substrates after imprinting. d, Photographs of the imprinted tissue and fluorescence microscopy images of the corresponding molecular replica. e, Quantification of replica-to-tissue area ratio. Data are mean ± s.e.m. (N = 3 independent biological replicates). Statistics: two-tailed unpaired t-test. f, Quantification of RNA, lipids and proteins from nanoneedle replicas with different surface chemistries (as-etched, APTES, oxidized) and a 10-µm brain tissue section. ata are mean ± s.e.m. (N = 3 independent biological replicates). Statistics: two-way ANOVA with a post-hoc Tukey’s multiple-comparisons test. g, DESI-MSI maps of grey matter (m/z 834.53, blue) and white matter (m/z 888.62, green) lipids from a tissue slice and molecular replicas generated using porous silicon nanoneedles (pSi nN), solid silicon nanoneedles (solid nN), porous silicon nanoneedles over a porous silicon layer (pSi nN + layer) and a porous silicon layer (pSi layer). h, Quantification of the number of features as a function of the confidence threshold set for peak identification in samples from g. Data are mean ± s.e.m. (N = 3 independent biological replicates). i, The feature count at a peak threshold of 2.0 (95% confidence) for samples from g. Data are mean ± s.e.m. (N = 3 independent biological replicates). j, The correlation matrix of spectra from three tissue sections and their corresponding replicas from g. k, The relative surface area of molecular replicas to original tissue for samples from g. Data are mean ± s.e.m. (N = 3 independent biological replicates). Statistics: ordinary one-way ANOVA with post-hoc Tukey’s multiple-comparisons test. l, A correlation matrix comparing three pSi nN replicas with the original tissue section. m, A correlation scatter plot of lipids peak intensities between the tissue section and the first replica. n, A correlation scatter plot of lipid peak intensities between the first and second replica.
