Fig. 3: Spatial lipidomics of murine glioma replicas.
From: Nanoneedles enable spatiotemporal lipidomics of living tissues
a, HCA maps of the top two clusters from the DESI-MSI data of a murine brain section and its molecular replica, corresponding to grey matter (blue) and white matter (yellow). b, Average spectra displaying normalized counts versus m/z, for the top two clusters associated with grey matter (GM) and white matter (WM) in section (S) and replica (R). c, Volcano plots of the differential lipid distribution between grey and white matter, highlighting the role of m/z 834.53 (PS 40:6) and 888.62 (SHexCer 42:2;O2) as grey and white matter biomarkers in both section and replica. d, An overlay map of TIC-normalized intensity for m/z 834.53 (blue) and 888.62 (yellow). e, HCA maps of the top three clusters from the DESI-MSI dataset of a tumour-bearing mouse brain section and replica, corresponding to grey matter (GM, blue), white matter (WM, yellow), and tumour (T, red). White boxes indicate regions used for lipid abundance analysis. f, Average spectra showing normalized counts versus m/z, for the three clusters associated with grey matter (GM), white matter (WM) and tumour (T) in section (S) and replica (R). g, Volcano plots comparing lipid distribution between GM and T, highlighting the most representative lipid peaks in both section and replica. h, An overlay map of cumulative TIC-normalized intensity for peaks marking grey matter (m/z 834.53 and m/z 600.51, blue), white matter (m/z 844.64, m/z 860.64 and m/z 888.62, yellow) and tumour (m/z 682.59, m/z 716.52 and m/z 736.65, red) in section and replica. The white boxes indicate regions used for lipid abundance analysis across GM, WM and T. i,j, Heatmaps showing the relative abundance of 33 lipid peaks (from analysis in g) along the major axis of the white box in e and h for the section (i) and replica (j). Top and bottom strips above each heatmap show the cluster assignment and relative lipid intensity for corresponding pixels from e and h.
