Fig. 4: Spatial lipidomics of HG replica.
From: Nanoneedles enable spatiotemporal lipidomics of living tissues
a, A bright-field image of the haematoxylin and eosin (H&E)-stained section of HG biopsy, showing infiltrated tumour (I), bulk tumour (T) and necrosis (N). b,c, HCA maps of the top three clusters from DESI-MSI images of the section (b) and replica (c). d, Volcano plots showing the differential lipid abundance analysis across I, T and N. consistently identifying m/z 600.51 (Cer 36:1;O2) for I, m/z 888.62 (SHexCer 42:2;O2) for T and m/z 680.54 (Cer 43:0;O3) for N in section and replica. e, A heatmap of the ten most representative lipids defining I, T and N in both section and replica. f, An overlay map of TIC-normalized intensity for m/z 600.51 (Cer 36:1;O2, red), 888.62 (SHexCer 42:2;O2, blue) and 680.54 (Cer 43:0;O3, yellow).
