Fig. 2: Detection of nucleotide mutations on engineered nanolatches.
a, A sketch of the MS2 carrier design and sequence comparison between the complementary nanolatch (MH) and its six mutated variants (MHm1–MHm6), highlighting nucleotide changes in both pairing and competitive areas that interact with the target site on the MS2 carrier. b, The mechanism of the competitive interaction between the MS2 RNA target site and nanolatches/complementary DNA oligo, illustrated using the fully complementary nanolatch MH and single-nucleotide mutated nanolatch MHm1. The representative nanopore current traces exhibit distinct signatures corresponding to the ‘loop latched’ (positive event) and ‘loop unlatched’ (negative event) states. The positive event ratio is defined as the number of positive events divided by the total number of events per measurement (n = 100). c, An analysis of positive event ratios for nanolatches MH–MHm6. The bar charts present positive event ratios from three independent measurements (N = 3) for each nanolatch. The data are shown as the mean ± standard deviation.
