Fig. 5: Detection of m5C modification on E. coli 16S rRNA compared with A. baumannii 16S rRNA.
a, A modification map of E. coli 16S rRNA, highlighting the essential role of the methyltransferase RsmF in catalysing methylation at cytosine 1407. b, The sequences of E. coli and A. baumannii 16S rRNA at the target site. The cytosine at position 1407 is methylated in E. coli 16S rRNA but remains unmethylated in A. baumannii 16S rRNA. c, A schematic of the E. coli/A. baumannii 16S rRNA carrier design, featuring a reference structure on one side of the carrier and the target site at the opposite end. d, Representative nanopore current traces of the E. coli/A. baumannii 16S rRNA carrier. e, The analysis of the positive event ratio for E. coli and A. baumannii 16S rRNA carriers using the same nanolatch. The carrier concentration was maintained at 0.3 nM and the nanolatch concentration at 3 nM. The higher positive event ratio observed for E. coli can be attributed to the stabilizing effect of the m5C modification at the target site. The data are shown as mean ± standard deviation from three independent measurements.
