Extended Data Fig. 2: Verification of the nanolatch design using bulk methods.
a, Schematic of the nanolatch design incorporating fluorophore and quencher pairs, independent of the MS2 carrier context. The positive control nanolatch (MH) induces loop formation by connecting the two ends of the linear complex, bringing the fluorophore and quencher into close proximity and resulting in a non-fluorescent ‘Fluorescence OFF’ state. In contrast, the negative control nanolatch (MT) fails to consistently latch the loop, allowing fluorescence emission and maintaining a ‘Fluorescence ON’ state. b,c, Fluorescence measurements comparing the MT and MH nanolatches. The significantly reduced fluorescence intensity observed for the fully complementary MH nanolatch, relative to the mismatched MT nanolatch, demonstrates both the selectivity (b) and rapid kinetics (c) of loop formation. d, Agarose gel electrophoresis analysis confirming loop formation using the nanolatch strategy. A distinct loop complex band is observed only with a fully complementary nanolatch.
