Extended Data Fig. 3: Optimization of pairing and competitive area lengths at the target site. | Nature Nanotechnology

Extended Data Fig. 3: Optimization of pairing and competitive area lengths at the target site.

From: Nanopore detection of single-nucleotide RNA mutations and modifications with programmable nanolatches

Extended Data Fig. 3

a, Schematic of the MS2 carrier design. b, Design parameters for each target site, including pairing and competitive area lengths and the corresponding nanolatch sequences. c, Nanopore measurement results for all target site designs, each measured with a fully complementary and a mismatched nanolatch, respectively. The greatest discrimination between non-mismatched and mismatched sequences is observed with a pairing area of 10 nt and a competitive area of 8 nt. Data are presented as mean ± standard deviation from three independent measurements.

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