Extended Data Fig. 6: Unique site-specific electrophysiological signals when recording from a different region than where sonication was applied.
a. Electrophysiological signals were recorded from either the mPFC or RsC after treatment with SonoKet and sonication either at the mPFC or the RsC. Spectrograms depicting electrophysiological response to SonoKet (0.75 mg/kg) with recording from the mPFC while ultrasound is applied to the mPFC (top left; n = 5; repeated from Fig. 4) or RsC (top right; n = 6); and recording from the RsC while ultrasound is applied to the mPFC (bottom left; n = 5) or RsC (bottom row; n = 5; repeated from Fig. 4). Power spectral density percent changes from the baseline (0-5 mins) were calculated. b. Average percent change in power spectral density from baseline versus frequency for each of the three indicated time periods for each recording site and treatment: time of sonication (7.5-10 min), time immediately after treatment (10-25 min), and in a delayed period (25-45 min). c. Power spectral density percent change from baseline area under the curve for each spectral band: theta (4-8 Hz), alpha (8-15 Hz), beta (15-25 Hz), and gamma (25-55 Hz). Percent changes were calculated for each of the three indicated time periods. Data presented as box plots. Unpaired t-test, *p < 0.05, **p < 0.01.
