Extended Data Fig. 1: Statistics of droplet patterned microtubules imaged with TIRF microscopy.
From: A hydrodynamic instability drives protein droplet formation on microtubules to nucleate branches
a, Histogram of droplet sizes and spacings for TIRF experiments at 1 μM GFP–TPX2. N = 35 microtubules were analyzed with a mean size of 0.5 ± 0.1μm and spacing 0.6 ± 0.2μm (mean ± standard deviation). b, Average power spectrum of GFP–TPX2 fluorescence intensities of droplet patterns for TIRF experiments at 1 μM GFP–TPX2 (N = 35 microtubules). The peak indicates the emergence of a periodic pattern with wavelength \({\lambda }_{\max }=0.6\pm 0.1\,\mu {\rm{m}}\) (mean ± standard deviation), in agreement with the histogram analysis. Shaded regions are 95% bootstrap confidence intervals. For calculation details, see Methods.
