Extended Data Fig. 6: Effects of Rac1, Arp2/3 and myosin II inhibitions on rotating cell trains.
From: The role of single-cell mechanical behaviour and polarity in driving collective cell migration
a, PBD signal distribution before (top) and after (bottom) addition of the Rac1 inhibitor Z62954982 (100 µM) on rotating rings. b, Intensity profile of PBD signal in entire rings before (top) and after (bottom) drug treatment. Expanded views (marked in red box) showing the disappearance of a single cell PBD signal gradient after drug addition. c, Displacement kymograph of the PBD images traced along the circumferential midline of the ring track; the white dashed line indicates drug addition. d, e, Coordination parameters and speeds measured before and after Rac1 inhibition. Black dashed lines indicate drug addition (n=5, m=2). f, Coordination parameters evolution before and following Arp2/3 inhibition (CK666, 100 µM, n=37) and myosin II inhibition (Blebbistatin, 80 µM, n=30). Dashed vertical lines (blue and red color) indicate the point of drug addition respectively. All error bars indicated are standard deviations. All scale bars: 50 µm.
