Extended Data Fig. 2: Repolarization time scale of single cell and single cell polarities.
From: The role of single-cell mechanical behaviour and polarity in driving collective cell migration
a,b, Spatio-temporal montage of MDCK-PBD-YFP cells showing repolarization of a single cell upon collision. White arrows (dashed) show the direction of cell migration. Red arrowheads indicate the time of the collision (at 2 min.) and the appearance of Rac1 repolarization signal (at 6 min.), respectively. c, Confocal image of basal plane in a 50 µm wide ring shows a PBD gradients in all the cells indicating cell scale polarized Rac1 activities. d, Line intensity profile (as visualized by PBD biosensor intensity) shows average Rac1 gradient of single cells (n=42 from 2 rings, m=2) from multiple rings (as represented in a single cell marked by line ab in panel c). e, MDCK cells expressing YFP–PBD and mcherry-AHPH. f, Saptio-temporal displacement kymograph shows the single cell (marked with white dashed line) retain the front–rear characteristics during rotations. g, Distribution of AHPH (red) and PBD (green) at single cell level from the orthogonal view at one time point. Typical example of a single cell with boundaries marked in dashed white line (XZ scale bar: 2 µm). h, Line graph showing the intensity distribution of PBD and AHPH in single cells (n=5 cells) of a rotating ring. i, Schematics showing the gradient of PBD and AHPH in single cells of a train. All error bars indicated are standard deviations. All scale bars unless mentioned specifically: 50 µm.
