Extended Data Fig. 3: Immunostainings of cell–substrate adhesions (paxillin) and cell–cell adhesion (E-cadherin).
From: The role of single-cell mechanical behaviour and polarity in driving collective cell migration
a, Immunofluorescence for E-cadherin-GFP (Z-projection) in a rotating ring. Anti-clockwise direction is indicated by the white dashed arrows. The diffused E-cadherin distribution indicates the cryptic lamellipodia (indicated by white arrows). b, Basal immunofluorescent paxillin staining labelled focal adhesions. c, Nuclei labelled in blue. d, Merge E-cadherin (green), paxillin (red) and nuclei (blue). e-f, Images showing enlarged views of (e’-f’). g, Schematic showing single cells in rotating ring with cryptic lamellipodia revealed by polarized distributions of E-cadherin and paxillin. Scale bars: 50 µm (a-d), 20 µm (e, f).
