Extended Data Fig. 2: Genetic and physical perturbations of germ cells.
From: A hydraulic instability drives the cell death decision in the nematode germline
A-C, Representative ced-3(RNAi) gonads. A, PIV on-axis speed (color-code) overlaid on a central cross section of a gonad expressing LifeAct::mKate treated with ced-3(RNAi). B, Maximum intensity projection of an adult gonad treated with ced- 3(RNAi), where the cell membrane expressing marker mCherry::PH-domain was imaged. C, 3D rendering of a representative ced-3(RNAi) germline where germ cells were segmented. Color code indicates germ cell volume. D, Open circles, rachis flux Qr for the L4440 control RNAi from 10 gonad arms. Solid line, theory fit (see Supplementary Information). E, Standard deviation of cell volumes plotted against average cell volume for non-RNAi and ced-3(RNAi) germlines. F, Distribution of germ cell volumes located between 85%-100% gonad length treated with ani-2(RNAi) for 24 hours; solid curves indicate superposition of two Gaussian distributions; vertical dash line indicates crossover volume of 150 fL. G, Fraction of germ cells smaller than 150 fL located at proximal end (85%-100% gonad length) for non-treated control and ani-2(RNAi) condition. H, Number of germ cell corpses per gonad arm under L4440 control(RNAi) (n=2) and ani-2(RNAi) treatment (n=3), identified by characteristic apoptotic rounding (see Supplementary Fig. 3). I, Relative volume change of germ cells during the approximately 15 min of thermoviscous pumping, for the FLUCS CTRL and FLUCS OUT experiment (Fig. 3c). Scale bars, 20 μm. Error bars represent error of the mean at 95% confidence.
