Extended Data Fig. 7: Nuclear orientation and YAP nuclear export are modulated by substrate concave curvatures.
From: Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation
Mean orientation of the nuclei on (A) convex (crest) and (B) concave (valley) curvature zones of λ20 (in grey), λ30 (in red) and λ50 (in blue) wavy hydrogels. All data are shown as mean ± SD. The number of nuclei is indicated at the bottom of each bar: 170 ≤ n ≤ 368 for concave curvature and 109 ≤ n ≤ 405 for convex curvature. Nuclear to cytoplasmic YAP ratio of nuclei on interm., concave, convex zones of (C) λ20 in grey, (B) λ30 in red and (C) λ50 in blue corrugated hydrogels. Black bars correspond to flat hydrogels. For λ20 n=30 (interm.), n=16 (concave), 24 (convex) and n=50 (flat) obtained from 5 to 7 replicates, for λ30 n=18 (interm.), n=18 (concave), n=9 (convex) and n=50 (flat) obtained from 5 to 9 replicates and for λ50 n=6 (concave), n=7 (convex) and n=50 (flat) obtained from 3 to 5 replicates. All data are shown as mean ± SD. *p < 0.1, **p < 0.01, ***p < 0.001, ****p < 0.0001 and n.s. not significant.
