Fig. 3: Two-state heterogeneous model of prewetting on DNA captures the sequence-dependent switching of Klf4 to a condensed state.

a, Schematic of the two-state heterogeneous model of prewetting (Supplementary Note). The DNA is considered as a one-dimensional lattice of sites that can be in either one of the two states, adsorbed state (s_i = –1) or condensed state (s_i = +1). b, DNA sites have an inhomogeneous propensity for condensation (denoted by h_i; Supplementary Note) given by the binding energy landscape inferred from the position weight matrix (Fig. 2g and Extended Data Fig. 9a, bottom). c, Average experimental kymograph ([Klf4-GFP]: 105–281 nM, N = 34; top) shows a spatial localization pattern consistent with the coarse-grained binding energy landscape (middle, colour map representation of h_i; Fig. 2h). The average kymograph obtained from the model (N = 400; bottom) captures this pattern. The spatial dimension of the model kymograph was convolved with a Gaussian function with a full-width at half-maximum corresponding to that of the point spread function of the microscopy system (Extended Data Fig. 3c). The unconvolved average kymograph and individual kymographs are shown in Extended Data Fig. 9f,g.