Extended Data Fig. 6: MDA-MB-231 cells spread rapidly in response to increased viscosity despite perturbations to membrane availability and passive membrane reservoirs.
From: Membrane ruffling is a mechanosensor of extracellular fluid viscosity
A) Cells treated with 80 μM dynasore to suppress endocytosis underwent rapid spread area increases when exposed to 1% MC. Envelope: SEM; N = 22. B) Cell spread area did not change in response to hypertonic treatment (530 mOsm) and then increased in response to 1% MC following hypertonic treatment. Envelope: SEM; N = 23. C) Following hypotonic treatment (160 mOsm), which led to a small decrease in cell spread area, cell spreading in 1% MC was slowed slightly due to increased membrane tension; spreading increased substantially when cells were exposed to 2% MC. Envelopes: SEM; N = 27 (hypotonic 1% MC), 27 (hypotonic 2% MC).
