Extended Data Fig. 1: Characterization of viscous media and cellular responses to increased viscosity.
From: Membrane ruffling is a mechanosensor of extracellular fluid viscosity
a) Viscous solutions were measured using a rheometer; shown are viscosity vs. shear curves for various media used in this study. b) Viscosity of each solution measured at 37o C at 100 Hz shear. c,d) Viscous medium does not significantly affect cell persistence in c) MDA-MB-231 cells or in d) NIH 3T3 fibroblasts. Direction autocorrelation was calculated using DiPer3; in c) N = 305, 370, and 176 for DMEM, 1% MC, and 1% PEO, respectively; in d) N = 120, 120, and 102 for DMEM, 1% MC, and 1% PEO, respectively; error bars: SEM. e,f) Viscous medium induced speed increases across cell types. e) Treating RAW 264.7 macrophages with 1% MC led to an increase in cell speed. Error bars: SD; p < 0.0001 (****); N = 37 (DMEM), 51 (1% MC). f) Treating AG19642 non-wound healing human fibroblasts with 1% MC led to an increase in cell speed. Error bars: SD; p < 0.001 (***); N = 64 (DMEM), 57 (1% MC).
