Extended Data Fig. 2: Cells became flatter as they spread in viscous medium.
From: Membrane ruffling is a mechanosensor of extracellular fluid viscosity
a) Time lapse xz-slices showed MDA-MB-231 cells, labelled with eGFP-F-tractin, flattening in viscous medium. 1% MC added at 1 minute; scale: 10 μm. b) Cell height was measured 4 μm towards the cell center from the leading edge. Error bars: SD; p ≥ 0.05 (n.s.), < 0.01 (**); N = 4 cells. c) Representative images of an MDA-MB-231 nucleus xz-cross-section stained with Hoescht showing nuclear shape change in viscous medium; 1% MC added at 1 minute; scale: 10 μm. d) Nuclear height decreased, e) nuclear width increased, and f) nuclear volume decreased slightly after MDA-MB-231 cells were treated with 1% MC at 1 minute; error bars: SD; significance reported with respect to DMEM group; p ≥ 0.05 (n.s.), < 0.1 (*), < 0.01 (**), < 0.001 (***), < 0.0001 (****); N = 12 cells.
