Extended Data Fig. 3: Characterization of the role of integrin and myosin II in cell spreading and traction force generation in response to increased viscosity.
From: Membrane ruffling is a mechanosensor of extracellular fluid viscosity
a) Representative images of an MDA-MB-231 cell on a PLL-coated substrate show no spreading following the addition of 1% MC. Scale: 20 μm. b,c) Both the b) maximum traction stresses and c) total traction forces increased in 1% MC for both untreated and blebbistatin-treated cells. Error bars: SD; p ≥ 0.05 (n.s.), < 0.05 (*); < 0.01 (**); N = 15 cells for each condition. d) Representative SIM images of myosin IIA staining in MDA-MB-231 cells after 30 minutes incubation in 1% MC show the formation of myosin stacks. Scale: 10 μm.
