Fig. 5: Extent of cell rearrangements depends on proliferation rate.
From: Cell cycle dynamics control fluidity of the developing mouse neuroepithelium
a, Laser ablation of apical junctions at E8.5 and E10.5. Apical view, anterior left, dorsal up. The laser cut was performed at t = 0 s along the yellow line. The vertex positions (asterisks) were tracked to measure the recoil velocity. Scale bars, 10 μm. b, Mean displacement of the vertices over time. Error bars, 95% CI. c, Initial recoil velocity of vertices after laser ablation at the indicated stages (Methods). Mann–Whitney test; P > 0.05 (with and without outliers). Samples sizes in b and c are as follows: E8.5 (n = 14 ablations); E10.5 (n = 11 ablations) (Supplementary Table 1). 25–75 percentile (box), median (blue), mean (red), highest/lowest observations without outliers (whiskers). d, Left: fragmentation coefficient (ϕ) for simulations with low proliferation rate kp = 0.03 h−1. Right: difference in ϕ between high (0.09 h−1; Fig. 3a) and low (0.03 h−1) kp. e, Confetti clones (red) induced at E7.5, embryos cultured from E8.5 for 42 h with vehicle or 210 μM l-mimosine. ZO1 immunostaining, white. Scale bars, 10 μm. f, Clone size quantification for e. Mann–Whitney test (two sided); *P = 0.019. Box plots as in c. Sample sizes are as follows: control (n = 382 clones); l-mimosine (n = 155 clones). g, Mean number of fragments per clone for the experiment in e and f. Corresponding fragmentation coefficient ϕ (95% CI) was obtained using a linear fit to the data for clones ≤8 cells (dashed lines). Error bars, s.e.m.; sample size as shown in f.
