Extended Data Fig. 4: Dimensions of laser ablation experiments performed in metaphase and the PNC stage.

Centrosomes are presented by green balls in all 3D schematics. Centrosomes and astral MTs are sketched in green and chromosomes in magenta in all 2D schematics. All ablation geometrics are portrayed in red. The A–P directions are indicated by brown arrows. a,b, Metaphase: dimensions of the rectangular plane cuts in Fig. 4a,b respectively. c,d, Metaphase: dimensions of the cup cuts in Fig. 4c,d respectively. e, The PNC stage: dimensions of the rectangular plane cuts in Fig. 5a,b. f,g, The PNC stage: the plane cuts near the anterior or leading centrosomes (f), and the plane cuts near the posterior or trailing centrosomes (g). Both upper parts are the schematics showing the definition of centrosome centre displacement d, which is the relative orthogonal (away from the plane cut) displacement from the locus right before cutting. The black arrows indicate the progression of pronuclear migration and rotation. The mean displacement results with SEM error bars, including uncut and cut embryos, are shown in both lower parts. Their raw data are plotted semi-transparently in the back layer. Sample numbers are f, n = 25 (uncut), n = 25 (with cut) and g, n = 23 (uncut), n = 23 (with cut). And the data in Fig. 5b is the combination of these two data sets (f and g), including both plane cuts near the leading and the trailing centrosomes. h, The PNC stage: dimensions of the large semicircular arc cuts (near anterior cortices) in Fig. 5f–h. All scale bars on images are 10 μm.