Extended Data Fig. 5: Parameter sweeping simulations of the cortical pulling model.

a-c, The examples of simulated centrosome movements while parameter sweeping. a, How varying the pulling force f₀ per force generator affects the PNC centration and the corresponding exponential fitting of τ_c. b, How varying the motor number M in metaphase affects the stable centring behavior under the condition of exerting an external force for 150 seconds and releasing. The centrosome motion is shown with the corresponding exponential fitting of τ_f and the spring constant k_s calculated from the theory. c, How changing the cortical motor detachment rate κ affects the spindle oscillations. The corresponding M_c were derived from the theory and we adopted the same motor number (above all M_c) to simulate the oscillations. The averaged oscillation frequencies and amplitudes are specified. d-h, The results of PNC centration (τ_c), metaphase stable centring (τ_f and spring constant k_s), and anaphase oscillation (M_c, and oscillation frequency and amplitude) under different parameters.