Extended Data Fig. 4: Bulk cytoplasmic flows during CP formation. | Nature Physics

Extended Data Fig. 4: Bulk cytoplasmic flows during CP formation.

From: Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization

Extended Data Fig. 4

A, Brightfield images (cross-sections) of a representative oocyte at consecutive timepoints during CP morphogenesis and PIV analysis of bulk cytoplasmic flows (yellow arrows). Scale bar, 30 µm. B and B’, Kymographs of bulk cytoplasmic flow velocities along the oocyte AV axis as a function of time after fertilization (0 min, dashed horizontal line) analyzed in a region perpendicular (B) and parallel (B’) to the oocyte AV axis. Colour bar indicates velocity in µm min−1 (positive velocities towards the AP, negative velocities towards the VP). B”, Violin plots of the average velocity of bulk cytoplasmic flows, measured in areas parallel (green, N = 3 animals, n = 4 oocytes) and perpendicular to the oocyte AV axis (blue, N = 3 animals, n = 4 oocytes), and cortical actin flows along the oocyte AV axis (red, N = 4 animals, n = 4 oocytes). Solid lines indicate median. Dashed lines indicate quartiles. One-way ANOVA test with’Tukey’s multiple comparisons test. Unfertilized: P = 0.7841 for AV axis-LR axis, P = 0.6321 for AV axis-Actin and P = 0.6553 for LR axis-Actin; 1mpf: P = 0.7270; *P = 0.0227; **P = 0.0071; 3mpf: P = 0.9401; *P = 0.0412 for AV axis-Actin and *P = 0.0244 for LR axis-Actin; 6mpf: P = 0.0596; ***P = 0.0002; **P = 0.0068; 9mpf: P = 0.9871 for AV axis-LR axis, P = 0.4320 for AV axis-Actin and P = 0.3581 for LR axis-Actin; 13mpf: P = 0.4983 for AV axis-LR axis, P = 0.3306 for AV axis-Actin and P = 0.9382 for LR axis-Actin. ns, not significant.

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