Extended Data Fig. 5: Surface tension of the unfertilized oocyte along the AV axis.
A, Violin plot of the maximum expansion/opening of the actin cortex at the animal pole following UV-laser ablation. N = 3 animals, n = 3 oocytes. Solid line indicates the median, dashed lines indicate the quartiles. B, Violin plots of the surface tension at the AP (N = 6 animals, n = 9 oocytes) and VP (N = 5 animals, n = 12 oocytes) of unfertilized oocytes. Solid lines indicate the median, dashed lines indicate the quartiles. Two-sided Mann-Whitney test (**P = 0.0056). C, Violin plots of the surface tension at the VP of unfertilized oocytes determined with different pipette diameters: 10 µm (N = 3 animals, n = 4 oocytes), 20 µm (N = 4 animals, n = 7 oocytes), 35 µm (N = 4 animals, n = 10 oocytes), 40 µm (N = 4 animals, n = 10 oocytes). Solid line indicates the median, and the dotted line indicates the quartiles. Kruskal-Wallis test with Dunn’s multiple comparison test (ns, not significant, P > 0.9999 for 10 µm-20 µm, 10 µm-35 µm, 10 µm-40 µm, 20 µm-35 µm, 20 µm-40 µm and 35 µm-40 µm; P = 0.1403 for 35 µm-40 µm). D, Oocyte shape model prediction of a bulge near the VP associated with the steep gradient in cortical actin density. Color bar indicates actin density in arbitrary units. See text for choice of model parameters. E, Plot of the AR during CP initiation and expansion predicted by the model with friction \(\xi =1.0\eta {R}^{2}\). See text for choice of model parameters.
