Extended Data Fig. 8: Oocyte centrifugation alters the position of the compacted myoplasm.
A, Schematic representation of the centrifugation experiment. Taken from https://doi.org/10.15479/at:ista:9623. B, Violin plots of the diameter of control (blue bar. N = 5 animals, n = 11 oocytes) and centrifuged unfertilized oocytes (pink bar. N = 5 animals, n = 10 oocytes). Solid lines indicate median. Dashed lines indicate quartiles. Mann-Whitney test (n.s. is not significant, P = 0.1366). C, Confocal cross-sections of control and centrifuged oocytes immunostained for myoplasm (mouse anti-neurofilament antibody - see methods; blue), endoplasmic reticulum (rabbit anti-phospho-S6 antibody - see methods; magenta), and DNA (Hoechst; orange, merge). Arrowheads point at the displacement of the myoplasm from the vegetal-most cortex. Scale bar, 30 µm. D, Plot of total actin intensity in the animal (AP) and vegetal (VP) hemispheres of centrifuged oocytes as a function of time after fertilization (0 min, dashed vertical line). N = 3 animals, n = 3 oocytes. Error bars, s.e.m.
