Extended Data Fig. 2: Interfering with the actomyosin, but not microtubule cytoskeleton, affects oocyte shape changes. | Nature Physics

Extended Data Fig. 2: Interfering with the actomyosin, but not microtubule cytoskeleton, affects oocyte shape changes.

From: Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization

Extended Data Fig. 2

Maximum intensity projections of F-actin (green) and pMyosin (magenta) in fixed control oocytes and oocytes at different stages of CP morphogenesis exposed to DMSO (0.1%), injected with CaMypt mRNA ( ~ 0.3 nl of 1.5 µg µl−1 mRNA, myosin II inhibition), and treated with Nocodazole (0.6 µM, microtubule disruption) or LatB (1 µg µl−1; F-actin disruption). F-actin was detected by Phalloidin and pMyosin by immunohistochemistry. Note that, due to the lack of deformations in LatB-treated oocytes upon fertilization, fertilized LatB-treated oocytes could only be reliably identified from 3mpf onwards. Scale bar, 30 µm.

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