Extended Data Fig. 3: Cortical actomyosin dynamics during CP formation.
A, Confocal fluorescence images of sum intensity projections over 10 central z-slides of cortical actin (top row) and cortical myosin II (bottom row) distribution in a representative oocyte during CP morphogenesis. Arrowheads,vegetal bulge. Scale bar, 30 µm. B, Plot of the ratio of cortical to cytoplasmic intensities for actin (left y-axis, N = 5 animals, n = 6 oocytes, green) and myosin II (right y-axis, N = 5 animals, n = 6 oocytes, magenta) as a function of time after fertilization (0 min, dashed vertical line). Error bars, s.e.m. C Confocal fluorescence images of maximum intensity projections of cortical myosin II distribution in a representative oocyte during CP morphogenesis. Arrowhead, vegetal bulge. Scale bar, 30 µm. D, Plot of the total myosin II intensity in the animal (AP) and vegetal (VP) hemispheres of oocytes/zygotes (N = 5 animals, n = 8 oocytes) as a function of time after fertilization (0 min, dashed vertical line). Error bars, s.e.m. E, Plot of temporal cross-correlation analysis between actin (N = 3 animals, n = 3 oocytes) and myosin II (N = 3 animals, n = 3 oocytes) total intensities. Individual actin-myosin II cross-correlations (semi-transparent orange lines) and the average (opaque orange line) are shown. F, Kymograph of myosin II flow velocity along the AV axis as a function of time after fertilization (0 min, dashed horizontal line). Color bar indicates velocity in µm min−1 (positive velocities towards the AP, negative velocities towards the VP).
