Extended Data Fig. 9: Mechano-directionality in the nuclear import of mechanosensitive myocardin-related transcription factor (MRTFA).
From: Structural anisotropy results in mechano-directional transport of proteins across nuclear pores
(A) Representative confocal microscopy image gallery of U2OS doubly transfected with R16-Y9P-MRTFA-GFP – MRTFA-mCherry (left) and Y9P-R16-MRTFA-GFP – MRTFA-mCherry (right) at different time points after serum stimulation, scale bar 10 µm. The mCherry-tagged MRTFA is used for normalizing the nuclear import kinetics in each individual cell. (B) Normalized average time courses of the relative nucleus-to-cytoplasm localization of R16-Y9P-MRTFA and Y9P-R16-MRTFA. The average time course is normalized by the average nucleus-to-cytoplasm accumulation (Ke) of the co-translocating MRTFA-mCherry construct. (C) Associated normalized nuclear import rates, R16-Y9P-MRTFA (\({\widetilde{k}}_{I}\) = 0.90 ± 0.08, n = 35); Y9P-R16-MRTFA (\({\widetilde{k}}_{I}\) = 0.70 ± 0.06, n = 25), where \({\widetilde{k}}_{I}\) is the normalized import rate calculated as kI(GFP)/kI(mCherry). Significance levels for two-tailed Mann-Whitney non-parametric test **P ≤ 0.01, P = 4.45 × 10−3. All points and bar plots indicate mean ± SEM.
